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il 15  (R&D Systems)


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    Structured Review

    R&D Systems il 15
    Il 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il+15/Recombinant+Human+IL-15+Protein/bio_rxiv__64898__2026__04__21__719872-209-14-15
    Average 95 stars, based on 200 article reviews
    il 15 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Produced:

    Article Title: Modeling simian immunodeficiency virus (SIV) latency in primary rhesus macaque CD4 + T cells
    Article Snippet: Parallel cultures were left unstimulated or exposed to individual LRAs for 48 hours, then SIV Gag p27 was quantified in culture supernatants using a commercial ELISA kit (Zeptometrix) according to the manufacturer’s instructions. .. Each replicate compared SIV p27 produced by unstimulated controls to the following stimuli: SAHA (250nM; MilliporeSigma), recombinant human IL-15 (25ng/mL; R&D Systems), bryostatin-1 (25ng/mL; R&D Systems), recombinant rhesus macaque TNF-α (10ng/mL; R&D Systems), ionomycin (750nM; Research Products International), and T cell activation beads (0.6 beads/cell). ..

    Recombinant:

    Article Title: Modeling simian immunodeficiency virus (SIV) latency in primary rhesus macaque CD4 + T cells
    Article Snippet: Parallel cultures were left unstimulated or exposed to individual LRAs for 48 hours, then SIV Gag p27 was quantified in culture supernatants using a commercial ELISA kit (Zeptometrix) according to the manufacturer’s instructions. .. Each replicate compared SIV p27 produced by unstimulated controls to the following stimuli: SAHA (250nM; MilliporeSigma), recombinant human IL-15 (25ng/mL; R&D Systems), bryostatin-1 (25ng/mL; R&D Systems), recombinant rhesus macaque TNF-α (10ng/mL; R&D Systems), ionomycin (750nM; Research Products International), and T cell activation beads (0.6 beads/cell). ..

    Article Title: Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins
    Article Snippet: Naïve T cells were cultured in basal media comprised of X-VIVO 15 Serum-free Hematopoietic Cell Medium (Lonza) with 5% FBS (v/v) (VWR), 10 mM N -Acetyl-L-cysteine (Sigma-Aldrich) and 50 μM 2-mercaptoethanol (Gibco), or frozen for long-term storage in basal media, without FBS, supplemented with 10% DMSO. .. Isolated or thawed naïve T cells were kept in basal media for 24 h and then activated by adding Dynabeads Human T-Activator CD3/CD28 (1:1 bead-to-cell ratio, Gibco), 300 U/ml recombinant human IL-2 (Peprotech), 5 ng/ml recombinant human IL-7 (Peprotech) and 5 ng/ml recombinant human IL-15 (R&D Systems). ..

    Article Title: Inhibition of long-lived plasma cells, a critical role for Telitacicept treatment to systemic lupus erythematosus.
    Article Snippet: Background: Telitacicept (RC18, RemeGen, Ltd) is a novel human TACI-Fc fusion protein that combines B cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL).. RC18 has recently been approved in China for the treatment of systemic lupus erythematosus (SLE), demonstrating its safety and efficacy in the majority of the target population.. However, the unique pharmacological mechanism of RC18 has not yet been fully elucidated.

    Article Title: Modeling simian immunodeficiency virus (SIV) latency in primary rhesus macaque CD4 + T cells
    Article Snippet: CD4 + T cells were resuspended in Priming Media at 5 x 10^6-8 x 10^6 cells/mL and seeded in flat-bottom 48-well tissue culture plates (Corning) at 1 mL per well. .. Priming Media consisted of R10 supplemented with recombinant human IL-2 (10U/mL; R&D Systems), recombinant human IL-15 (10ng/mL; R&D Systems), and FOXO1 inhibitor AS1842856 (500nM; Sigma Aldrich). ..

    Article Title: Ergosterol Attenuates Palmitate-Induced Tenocyte Apoptosis Through the Suppression of ER Stress and Myocyte-Derived IL-15/METRNL-Mediated Crosstalk
    Article Snippet: .. Cells were exposed to palmitate (200 or 400 μM), EG (0–100 μM), 3-MA (5 mM), and/or rapamycin (200 nM) for 24 h. Recombinant human IL-15 and METRNL proteins were purchased from R&D Systems (Minneapolis, MN) and administered according to the manufacturer’s recommendations. .. Tenocytes were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma, St. Louis, MO) for 30 min at 37 °C to assess cell viability.

    Article Title: Ergosterol Attenuates Palmitate-Induced Tenocyte Apoptosis Through the Suppression of ER Stress and Myocyte-Derived IL-15/METRNL-Mediated Crosstalk.
    Article Snippet: .. Cells were exposed to palmitate (200 or 400 μM), EG (0–100 μM), 3-MA (5 mM), and/or rapamycin (200 nM) for 24 h. Recombinant human IL-15 and METRNL proteins were purchased from R&D Systems (Minneapolis, MN) and administered according to the manufacturer’s recommendations. ..

    Article Title: Activation phenotypes defined by the coordinated expression of activation markers discriminate TCR-mediated and bystander T cell responses
    Article Snippet: .. PBMC were cultured in the presence of 100 ng/ml recombinant human IL-2 (Miltenyi Biotec) or 100 ng/ml recombinant human IL-15 (R&D Systems). ..

    Article Title: Integrative omics and phase IIa clinical trial identify TNF as key node in autoimmune hepatitis.
    Article Snippet: Frozen TRM1 and a.a. CD8+ T cells were thawed, rested in RPMI at 4°C for 1 hour, and stained with fluorochrome-labelled CD4 and CD8 antibodies (BioLegend), and a viability dye (Thermo Fisher). .. Cells were stimulated with 10 ng/ml recombinant human IL-15 (R&D Systems or PeproTech as specified) or 100 U/ml recombinant human IL-2 in RPMI at 37°C for 15 minutes, fixed with 2% PFA at 37°C for 15 minutes, permeabilized with 100% methanol on ice for 30 minutes, and stained with pSTAT5 antibody (Invitrogen) for 1 hour at room temperature. ..

    Activation Assay:

    Article Title: Modeling simian immunodeficiency virus (SIV) latency in primary rhesus macaque CD4 + T cells
    Article Snippet: Parallel cultures were left unstimulated or exposed to individual LRAs for 48 hours, then SIV Gag p27 was quantified in culture supernatants using a commercial ELISA kit (Zeptometrix) according to the manufacturer’s instructions. .. Each replicate compared SIV p27 produced by unstimulated controls to the following stimuli: SAHA (250nM; MilliporeSigma), recombinant human IL-15 (25ng/mL; R&D Systems), bryostatin-1 (25ng/mL; R&D Systems), recombinant rhesus macaque TNF-α (10ng/mL; R&D Systems), ionomycin (750nM; Research Products International), and T cell activation beads (0.6 beads/cell). ..

    Isolation:

    Article Title: Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins
    Article Snippet: Naïve T cells were cultured in basal media comprised of X-VIVO 15 Serum-free Hematopoietic Cell Medium (Lonza) with 5% FBS (v/v) (VWR), 10 mM N -Acetyl-L-cysteine (Sigma-Aldrich) and 50 μM 2-mercaptoethanol (Gibco), or frozen for long-term storage in basal media, without FBS, supplemented with 10% DMSO. .. Isolated or thawed naïve T cells were kept in basal media for 24 h and then activated by adding Dynabeads Human T-Activator CD3/CD28 (1:1 bead-to-cell ratio, Gibco), 300 U/ml recombinant human IL-2 (Peprotech), 5 ng/ml recombinant human IL-7 (Peprotech) and 5 ng/ml recombinant human IL-15 (R&D Systems). ..

    Modification:

    Article Title: Inhibition of long-lived plasma cells, a critical role for Telitacicept treatment to systemic lupus erythematosus.
    Article Snippet: Background: Telitacicept (RC18, RemeGen, Ltd) is a novel human TACI-Fc fusion protein that combines B cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL).. RC18 has recently been approved in China for the treatment of systemic lupus erythematosus (SLE), demonstrating its safety and efficacy in the majority of the target population.. However, the unique pharmacological mechanism of RC18 has not yet been fully elucidated.

    Cell Culture:

    Article Title: Activation phenotypes defined by the coordinated expression of activation markers discriminate TCR-mediated and bystander T cell responses
    Article Snippet: .. PBMC were cultured in the presence of 100 ng/ml recombinant human IL-2 (Miltenyi Biotec) or 100 ng/ml recombinant human IL-15 (R&D Systems). ..

    Staining:

    Article Title: Integrative omics and phase IIa clinical trial identify TNF as key node in autoimmune hepatitis.
    Article Snippet: Frozen TRM1 and a.a. CD8+ T cells were thawed, rested in RPMI at 4°C for 1 hour, and stained with fluorochrome-labelled CD4 and CD8 antibodies (BioLegend), and a viability dye (Thermo Fisher). .. Cells were stimulated with 10 ng/ml recombinant human IL-15 (R&D Systems or PeproTech as specified) or 100 U/ml recombinant human IL-2 in RPMI at 37°C for 15 minutes, fixed with 2% PFA at 37°C for 15 minutes, permeabilized with 100% methanol on ice for 30 minutes, and stained with pSTAT5 antibody (Invitrogen) for 1 hour at room temperature. ..



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